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胆汁酸对南美白对虾的黄曲霉毒素B1的生物富集、解毒系统和生长性能的影响

Effects of bile acids on aflatoxin B1 bioaccumulation, detoxification system, and growth performance of Pacific white shrimp

作者：Chen Su, Yusong Lu, Jinbao Li, Yuxuan Wang, Luqing Pan, Mengyu Zhang.

发布期刊：Food Chemistry. 2022 Mar 1;371:131169.

评估了胆汁酸在凡纳滨对虾体内降低黄曲霉毒素B₁（AFB1）残留物和毒性的潜力。幼虾和仔虾分别饲喂添加0、0.05、0.15和0.25 g/kg的胆汁酸的饵料，预饲60天。随后饲喂10天含有AFB1（2000 μg/kg）的饵料，并在5个时间点（30、60、63、66和70天）收集每个处理组的15只对虾（每组3个样本）。所有参数均采用标准方法测定，准确性在90-110%之间。结果表明，胆汁酸降低了对虾中AFB₁残留物（检出限：0.01 μg/L，相对标准偏差<10%，回收率：92.1-96.8%）。胆汁酸增加了对虾对AFB1的解毒作用，并通过增加II相代谢和抗氧化系统，提高了对AFB1的解毒能力，降低了氧化应激产物的水平，避免了AFB1诱导的虾肉变质和对人类的健康风险（置信度为95%）。

The potential of bile acids (BAs) to reduce aflatoxin B1 (AFB1) residues and toxicity in Litopenaeus vannamei was evaluated. Both juveniles and subadults were treated with 0, 0.05, 0.15 and 0.25 g/kg BAs for 60 days followed by 10-d AFB1 exposure (2000 μg/kg), and fifteen shrimp (five shrimp were pooled into one sample, n = 3) from each treatment were collected at five time points (30, 60, 63, 66 and 70 d). All parameters were determined using accepted and standard methods with acceptable accuracy (recovery) of 90-110%. Results demonstrated that BAs reduced the AFB1 residues in shrimp (limit of detection: 0.01 μg/L, relative standard deviation < 10% and recovery: 92.1-96.8%). BAs increased the detoxification of AFB1 and decreased the levels of oxidative stress products by increasing Phase II and antioxidant systems, avoiding AFB1-induced deterioration of shrimp meat and health risks to human. The confidence level was 95%.

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